You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.

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As the CSI, you collected the handwritten note from the bank teller. You also collected a pen that the robber used to write the note when he entered the bank.

  1. Explain how you photograph, mark, measure, sketch, collect and preserve the evidence.
  2. Consider possible later laboratory testing that you may want to perform to match the items to a suspect.

Your submission should be 1-2 pages in length, framed by a cover page and references page.

 

You are working as a CSI analyst. You collected DNA at a crime scene that is from the suspect but do not have enough to run a test to determine who the suspect is. Please discuss what you would do to attain more of the DNA and how to test the DNA to determine who the suspect is.

 

answer is very simple, as the amount of DNA acquired at the crime scene is not enough , I shall use this small amount of DNA of amplify it . In order to amplify the DNA the technique to be used to PCR, polymerase chain reaction . In this process it has the following steps

Denaturation:

This step involves heating the reaction mixture to 94°C for 15-30 seconds. During this, the double stranded DNA is denatured to single strands due to breakage in weak hydrogen bonds.

Annealing:
The reaction temperature is rapidly lowered to 54-60°C for 20-40 secs. This allows the primers to bind (anneal) to their complementary sequence in the template DNA.

Elongation:
Also known at extension, this step usually occurs at 72-80°C (most commonly 72°C). In this step, the polymerase enzyme sequentially adds bases to the 3′ each primer, extending the DNA sequence in the 5′ to 3′ direction. Under optimal conditions, DNA polymerase will add about 1,000 bp/minute.so thnow you have the elongated part of DNA , so now we can take a sample of DaNA from the suspect, denature it and then do electrophoresis test to find out whether the both samples of DNA . I.e the sample we got at crime scene which we again amplified it and the sample from the suspect , if both the DNA match we shall get similiar pattern in electrophoresis

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